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Registro completo
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Biblioteca (s) : |
INIA Las Brujas. |
Fecha : |
21/02/2014 |
Actualizado : |
17/10/2019 |
Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
Autor : |
FERREIRA, V.; PIANZZOLA, M.J.; VILARÓ, F.; GALVÁN, G.; TONDO, M.L.; RODRÍGUEZ, M.V.; ORELLANO, E.G.; VALSS, M.; SIRI, M.I. |
Afiliación : |
VIRGINIA FERREIRA, Departamento de Biociencias, Facultad de Química, Universidad de la República, Montevideo, Uruguay; MARÍA J. PIANZZOLA, Departamento de Biociencias, Facultad de Química, Universidad de la República, Montevideo, Uruguay; FRANCISCO LUIS VILARO PAREJA, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; GUILLERMO GALVÁN, Departamento de Producción Vegetal, Centro Regional Sur, Facultad de Agronomía, Universidad de la República, Canelones, Uruguay; MARÍA L. TONDO, Instituto de Biología Molecular y Celular de Rosario (CONICET-UNR), Rosario, Argentina; Área Biología Molecular, Facultad de Ciencias Bioquímicas y Farmacéuticas, Universidad Nacional de Rosario, Rosario, Argentina; MARÍA V. RODRÍGUEZ, Área Biología Vegetal (CONICET), Facultad de Ciencias Bioquímicas y Farmacéuticas, Universidad Nacional de Rosario, Rosario, Argentina; ELENA G. ORELLANO, Instituto de Biología Molecular y Celular de Rosario (CONICET-UNR), Rosario, Argentina; Área Biología Molecular, Facultad de Ciencias Bioquímicas y Farmacéuticas, Universidad Nacional de Rosario, Rosario, Argentina; MARC VALSS, Center for Research in Agricultural Genomics, CSIC, IRTA, Barcelona, Spain; Department of Genetics, Universitat de Barcelona, Barcelona, Spain; MARÍA I. SIRI, Departamento de Biociencias, Facultad de Química, Universidad de la República, Montevideo, Uruguay. |
Título : |
Interspecific potato breeding lines display differential colonization patterns and induced defense responses after Ralstonia solanacearum infection. |
Fecha de publicación : |
2017 |
Fuente / Imprenta : |
Frontiers in Plant Science, 28 August 2017, volume 8, 1424. OPEN ACCESS. |
ISSN : |
1664-462X |
DOI : |
10.3389/fpls.2017.01424 |
Idioma : |
Inglés |
Notas : |
Article history: Received: 30 June 2017 / Accepted: 02 August 2017 / Published: 28 August 2017. |
Contenido : |
ABSTRACT.
Potato (Solanum tuberosum L.) is one of the main hosts of Ralstonia solanacearum, the causative agent of bacterial wilt. This plant pathogen bacteria produce asymptomatic latent infections that promote its global spread, hindering disease control. A potato breeding program is conducted in Uruguay based on the introgression of resistance from the wild native species S. commersonii Dun. Currently, several backcrosses were generated exploiting the high genetic variability of this wild species resulting in advanced interspecific breeding lines with different levels of bacterial wilt resistance. The overall aim of this work was to characterize the interaction of the improved potato germplasm with R. solanacearum. Potato clones with different responses to R. solanacearum were selected, and colonization, dissemination and multiplication patterns after infection were evaluated. A R. solanacearum strain belonging to the phylotype IIB-sequevar 1, with high aggressiveness on potato was genetically modified to constitutively generate fluorescence and luminescence from either the green fluorescence protein gene or lux operon. These reporter strains were used to allow a direct and precise visualization of fluorescent and luminescent cells in plant tissues by confocal microscopy and luminometry. Based on wilting scoring and detection of latent infections, the selected clones were classified as susceptible or tolerant, while no immune-like resistance response was identified. Typical wilting symptoms in susceptible plants were correlated with high concentrations of bacteria in roots and along the stems. Tolerant clones showed a colonization pattern restricted to roots and a limited number of xylem vessels only in the stem base. Results indicate that resistance in potato is achieved through restriction of bacterial invasion and multiplication inside plant tissues, particularly in stems. Tolerant plants were also characterized by induction of anatomical and biochemical changes after R. solanacearum infection, including hyperplasic activity of conductor tissue, tylose production, callose and lignin deposition, and accumulation of reactive oxygen species. This study highlights the potential of the identified tolerant interspecific potato clones as valuable genetic resources for potato-breeding programs and leads to a better understanding of resistance against R. solanacearum in potato.
© 2017 Ferreira, Pianzzola, Vilaró, Galván, Tondo, Rodriguez, Orellano, Valls and Siri. MenosABSTRACT.
Potato (Solanum tuberosum L.) is one of the main hosts of Ralstonia solanacearum, the causative agent of bacterial wilt. This plant pathogen bacteria produce asymptomatic latent infections that promote its global spread, hindering disease control. A potato breeding program is conducted in Uruguay based on the introgression of resistance from the wild native species S. commersonii Dun. Currently, several backcrosses were generated exploiting the high genetic variability of this wild species resulting in advanced interspecific breeding lines with different levels of bacterial wilt resistance. The overall aim of this work was to characterize the interaction of the improved potato germplasm with R. solanacearum. Potato clones with different responses to R. solanacearum were selected, and colonization, dissemination and multiplication patterns after infection were evaluated. A R. solanacearum strain belonging to the phylotype IIB-sequevar 1, with high aggressiveness on potato was genetically modified to constitutively generate fluorescence and luminescence from either the green fluorescence protein gene or lux operon. These reporter strains were used to allow a direct and precise visualization of fluorescent and luminescent cells in plant tissues by confocal microscopy and luminometry. Based on wilting scoring and detection of latent infections, the selected clones were classified as susceptible or tolerant, while no immune-like resistance response was identified. Typic... Presentar Todo |
Palabras claves : |
Bacterial wilt; Disease resistance; Latent infections; Plant breeding; Potato; Ralstonia solanacearum; Solanum commersonii. |
Asunto categoría : |
F01 Cultivo |
Marc : |
LEADER 03620naa a2200337 a 4500 001 1012685 005 2019-10-17 008 2017 bl uuuu u00u1 u #d 022 $a1664-462X 024 7 $a10.3389/fpls.2017.01424$2DOI 100 1 $aFERREIRA, V. 245 $aInterspecific potato breeding lines display differential colonization patterns and induced defense responses after Ralstonia solanacearum infection.$h[electronic resource] 260 $c2017 500 $aArticle history: Received: 30 June 2017 / Accepted: 02 August 2017 / Published: 28 August 2017. 520 $aABSTRACT. Potato (Solanum tuberosum L.) is one of the main hosts of Ralstonia solanacearum, the causative agent of bacterial wilt. This plant pathogen bacteria produce asymptomatic latent infections that promote its global spread, hindering disease control. A potato breeding program is conducted in Uruguay based on the introgression of resistance from the wild native species S. commersonii Dun. Currently, several backcrosses were generated exploiting the high genetic variability of this wild species resulting in advanced interspecific breeding lines with different levels of bacterial wilt resistance. The overall aim of this work was to characterize the interaction of the improved potato germplasm with R. solanacearum. Potato clones with different responses to R. solanacearum were selected, and colonization, dissemination and multiplication patterns after infection were evaluated. A R. solanacearum strain belonging to the phylotype IIB-sequevar 1, with high aggressiveness on potato was genetically modified to constitutively generate fluorescence and luminescence from either the green fluorescence protein gene or lux operon. These reporter strains were used to allow a direct and precise visualization of fluorescent and luminescent cells in plant tissues by confocal microscopy and luminometry. Based on wilting scoring and detection of latent infections, the selected clones were classified as susceptible or tolerant, while no immune-like resistance response was identified. Typical wilting symptoms in susceptible plants were correlated with high concentrations of bacteria in roots and along the stems. Tolerant clones showed a colonization pattern restricted to roots and a limited number of xylem vessels only in the stem base. Results indicate that resistance in potato is achieved through restriction of bacterial invasion and multiplication inside plant tissues, particularly in stems. Tolerant plants were also characterized by induction of anatomical and biochemical changes after R. solanacearum infection, including hyperplasic activity of conductor tissue, tylose production, callose and lignin deposition, and accumulation of reactive oxygen species. This study highlights the potential of the identified tolerant interspecific potato clones as valuable genetic resources for potato-breeding programs and leads to a better understanding of resistance against R. solanacearum in potato. © 2017 Ferreira, Pianzzola, Vilaró, Galván, Tondo, Rodriguez, Orellano, Valls and Siri. 653 $aBacterial wilt 653 $aDisease resistance 653 $aLatent infections 653 $aPlant breeding 653 $aPotato 653 $aRalstonia solanacearum 653 $aSolanum commersonii 700 1 $aPIANZZOLA, M.J. 700 1 $aVILARÓ, F. 700 1 $aGALVÁN, G. 700 1 $aTONDO, M.L. 700 1 $aRODRÍGUEZ, M.V. 700 1 $aORELLANO, E.G. 700 1 $aVALSS, M. 700 1 $aSIRI, M.I. 773 $tFrontiers in Plant Science, 28 August 2017, volume 8, 1424. OPEN ACCESS.
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INIA Las Brujas (LB) |
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Registro completo
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Biblioteca (s) : |
INIA Treinta y Tres. |
Fecha actual : |
21/02/2014 |
Actualizado : |
20/03/2020 |
Tipo de producción científica : |
Trabajos en Congresos/Conferencias |
Autor : |
AYALA, W.; BARRIOS, E.; VELAZCO, J.I.; BERMÚDEZ, R. |
Afiliación : |
WALTER FELIZARDO AYALA SILVERA, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; ETHEL BALOISA BARRIOS PIRIZ, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; JOSÉ IGNACIO VELAZCO DE LOS REYES, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; RAÚL ENRIQUE BERMÚDEZ COQUARD, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay. |
Título : |
El uso de nabos forrajeros en la alimentación estival de terneros y corderos en la región Este de Uruguay [Resumen expandido. |
Fecha de publicación : |
2008 |
Fuente / Imprenta : |
ln: Reunión del Grupo Técnico en Forrajeras del Cono Sur, Grupo Campos, 22, 2008, Minas, Uruguay Ayala, W.; Lezama, F.; Barrios, E.; Bemhaja, M.; Saravia, H.; Formoso, D.; Boggiano, P., ed. Bioma campos : Innovando para mantener su sustentabilidad y competitividad. Memorias. Minas (Uruguay): Grupo Campos, 2008. |
Páginas : |
p. 227 |
Serie : |
Contiene CD con ampliación de trabajos presente en Memorias |
ISBN : |
978-9974-38-260-2 |
Idioma : |
Español |
Notas : |
Instituto Nacional de Investigación Agropecuaria, Uruguay |
Thesagro : |
ALIMENTACIÓN; BOVINOS; BRASSICAS; CORDEROS; OVINOS; TERNEROS; URUGUAY. |
Asunto categoría : |
L51 Fisiología Animal - Nutrición |
URL : |
http://www.ainfo.inia.uy/digital/bitstream/item/5336/1/Congreso-2008-campos-Ayala.pdf
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Marc : |
LEADER 01118naa a2200277 a 4500 001 1032356 005 2020-03-20 008 2008 bl uuuu u00u1 u #d 020 $a978-9974-38-260-2 100 1 $aAYALA, W. 245 $aEl uso de nabos forrajeros en la alimentación estival de terneros y corderos en la región Este de Uruguay [Resumen expandido. 260 $c2008 300 $ap. 227 490 $aContiene CD con ampliación de trabajos presente en Memorias 500 $aInstituto Nacional de Investigación Agropecuaria, Uruguay 650 $aALIMENTACIÓN 650 $aBOVINOS 650 $aBRASSICAS 650 $aCORDEROS 650 $aOVINOS 650 $aTERNEROS 650 $aURUGUAY 700 1 $aBARRIOS, E. 700 1 $aVELAZCO, J.I. 700 1 $aBERMÚDEZ, R. 773 $tln: Reunión del Grupo Técnico en Forrajeras del Cono Sur, Grupo Campos, 22, 2008, Minas, Uruguay Ayala, W.; Lezama, F.; Barrios, E.; Bemhaja, M.; Saravia, H.; Formoso, D.; Boggiano, P., ed. Bioma campos : Innovando para mantener su sustentabilidad y competitividad. Memorias. Minas (Uruguay): Grupo Campos, 2008.
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